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发信人: feat (大愚弱智), 信区: Postgraduate
标 题: 我向外国学者的求助（原创）
发信站: 荔园晨风BBS站 (Mon May 24 21:08:18 2004), 站内信件
作为深圳大学一名研究生，除了给人研究以外，还要学会与国际上的学术界交流。
小弟尽管只上了一年研究生，但这方面起步并不晚。这一年来由于课题需要，
小弟就经常冒犯国外的同行。我跟他们的交流主要是EMAIL,内容是专业求助，偶尔也聊聊
天。我发现一点，就是大部分外国人都很友善，你问他什么，只要他有空他就回答什么。
这也给我一个启示，就是我有什么问题都应该向外求助，而且是必须要。下面我粘贴一些
跟国外学者学术交流的EMAIL.这里面有寒假前跟韩国教授要东西的，开题需要向外国学者
求助的，也有要文献的。我想重申一点，深大的研究生应该比内地的学校的研究生更多
的跟国外学者交流，因为我们在现代化的城市，信息更加发达，当然也不是说别人怎样
起码我知道中科院，清华北大就很多人写信向鬼老要东西的。我也是学他们的。
效果很有冲击力，每次我的求助都有答复，我希望大伙同道能多多这样干。:-))
1.课题组需要，要从韩国引进杂交瘤细胞株，目的是自己自足，可以生产单抗，省钱。
由于组里只有我一个懂cell培养，于是我就担负起这个联系任务，当然是以boss名义发
EMAIL：
Dear Dr Kyoung Yong Jeong
Thank you very much for your kind help to my postgraduate.
It is maybe due to the long-time transportation(the package had stayed
in post-office for over two days), the monoclonal antibodies do not
work as well as expected.
> We are now researching on some subjects in urgent need of large
quantities of monoclonal antibodies against the major mite allergens(Der p1,Der
p2,
Der f1,Der f2). I wonder if it is possible for you to provide us with the
hybridomas(as a gift, for cooperation, or for selling) . You and prof.Yong are
welcome
to visit ShenZhen university, mayde we can find the common interests. The
traveling and other expenses are, of course, to be covered by us. I am looking
forwarding to hearing from you.
> With best regards to you and prof.Yong!
韩国PROF答应带细胞株来深圳，但我还有一些具体细节不懂，于是给他发以下
一封紧急求助信：
Thank you firstly for the information of media you have sent to us.As
this is the first time for us to culture hybridomas,we will do enough
preparation before you arrive.So we will be very glad if you can offer
us more details about the culture of hybridomas. There are some
questions asked as follows.
1. How will you take the hybridomas for transport?As I know,there have
been two usual ways for transporting cells.I’d like to show you the
protocol which I find from internet. I wonder which method you’d like
to use,because we need to get well ready before your arrival. Here
goes the protocol:
SUPPORT PROTOCOL: PREPARING CELLS FOR TRANSPORT
Both monolayer and suspension cultures can easily be shipped in 25-cm2
tissue culture flasks. Cells are grown to near confluency in a monolayer
or to desired density in suspension. Medium is removed from monolayer
cultures and the flask is filled with fresh medium. Fresh medium is
added to suspension cultures to fill the flask. It is essential that the
flasks be completely filled with medium to protect cells from drying if
flasks are inverted during transport. The cap is tightened and taped
securely in place. The flask is sealed in a leak-proof plastic bag or
other leak-proof container designed to prevent spillage in the event
that the flask should become damaged. The primary container is then
placed in a secondary insulated container to protect it from extreme
temperatures during transport. A biohazard label is affixed to the
outside of the package. Generally, cultures are transported by
same-day or overnight courier.
Cells can also be shipped frozen. The vial containing frozen cells is
removed from the liquid nitrogen freezer and placed immediately on dry
ice in an insulated container to prevent thawing during transport.
2. Would you bring us more than two cultures?we are afraid we cannot
assure the cell viability after we handle this for the first time.
3. Would you bring some media(500or1000ml) for us or sell to
us?Because of the mad cow disease in USA,our country have already
imposed a ban on the import of any serum product from USA including
Gibco.We can’t buy it from company and have to change another brand.It
’s absolutely helpful if you can bring some media for us.Also,We intend
to use your media for the culture first and then gradually change the
media for our own. I think it’s good for the cell to adapt the new
condition.Do you think so?
4. Can you send us some protocols about the preparation of Mabs,
hybridomas culture and the purification of Mabs?We hope we can know more
about the details of the monoclonal antibodies you have made.
We are very pleased if you can show us the details about the question
above.
Dr Yong回了我EMAIL，万事具备，就等两位学者来深大啦！当然他们来两天，我就要
陪他们两天，一个是带他们游深圳，一个是请教他们实验技术。幸亏我恶补了一下专英
还可以在具体操作中跟他们谈得来
2.开题需要，遇到技术瓶颈，向英国专家写EMAIL求助，想不到他会如此热情洋溢！
Dear Martin Blythe:
Hello.i'm graduate student study in shenzhen ,a city of China.i'm so
exciting that I find you via internet.because of the need of research, i want
to predict the B cell epitope of the recommbinant protein of interest.this
protein will be expressed in Pichia pastoris,i know this system would remian
the B cell epitope as natively as possible.but this protein is special-at least
I think,it's a fusion protein combined from two antigen.this is my question:
can fusion antigen remian the B cell epitope for each of two antigen
respectively?
how to predict B cell epitope of this fusion antigen with high accuracy via
internet? i'm will be very appreciated if you can do me this favor.
他热情的回信让我感动：
Thank you for your interest in my work. Unfortunately there is no
accurate
method for predicting Linear B cell epitopes currently available via the
internet. Protein sequence profiling methods using Hydrophilicity,
Flexibility,
and Accessibility etc... do not work well. However if you choose to use
one of
these methods they can be found here: http://www.imtech.res.
in/raghava/bcepred/
If your intention is to successfully predict only 1 or 2 epitopes form
your
protein I would recommend trying to find protruding loop or turn
structures
located on the surface of the protein. If there is a known 3D
structure of your
protein in the PDB database this should be quite easy. Programs such
as DSSP or
STRIDE can analyze your 3D protein structure and tell you were these
regions
are and how accessible they are.
Available here:
http://bioweb.pasteur.fr/seqanal/interfaces/dssp-simple.html
http://bioweb.pasteur.fr/seqanal/interfaces/stride.html
If you do not have a known structure for your protein I would use a
secondary
structure prediction program such as JPRED or PREDATOR to predict the
location
of loop and turn structures within your protein
http://www.compbio.dundee.ac.uk/~www-jpred/
http://bioweb.pasteur.fr/seqanal/interfaces/predator-simple.html
Once you have found a region of your protein that you think is likely to
contain a B cell epitope I would also recommend synthesising a large
peptide of
approximately 15 amino acids to cover it as the local secondary
structure maybe
important in enabling an antibody to cross-react.
Finally, I do not know a great deal about recombinant protein technology
but I
would imagine that so long as a predicted B cell epitope is still
accessible
and is not masked from being fussed to another protein (i.e. The
predicted
epitope is not facing the join to the other protein) the fussing or your
protein should not have a significant effect.
I hope I have been of some help to you,
回来我发信致谢，他也回了我：
Glad to be of help, let me know how your experiment turns out. Feel free
to ask me any questions you may have in the future that you think I might
be able to answer. I am currently working on developing new Linear B cell
epitope prediction methods but these will not be available for a few months
(that is if they work at all).
Martin Blythe.
友善的一个人，这是我结论。
3.要文献，大家也知道，有些杂志要钱，深大图书馆也没连接，那只好要了，一般他们都
会给，以下我给出一些模板：
Dear Dr. (author name)
I would appreciate receiving a reprint of your article: ********(不必全
写),杂志名. However, this Journal is not available in our library.
Thank you very much for your consideration.
Respectfully yours,
Yourself name
或者：
Dear Sir(or Pro):
I have read the abstract of your publication (add the name of the
article your ask for ) in the PubMed and I find it extremely useful
for my research .Unfortunately,I have not the access to the full-text
file and I am wondering if you could send it to me by E-mail via this
address.
Thank you indeed and the best wishes to you.
the best regard :your name
或者罗嗦一点的：
Dear Prof.(Dr.) XXX, (author's name)
I am a graduate (doctor) student majored in XXX (你的专业） of XXX
University （你的学校） in China. Recently, I found one of your
articles, titled "XXXXXXXXXXXX" （你所要的文章名）in "XXXX"(杂志). I
found it may help me achieve my goals in this research field. This would
make a really positive contribution to my work.
I would like to be able to read the full text of this article. The
abstract makes the article sound very interesting. I know there is usually a
fee required to obtain the full article from "XXXXXXXX"(杂志); however, as a
student, my only income is a small scholarship which is about US $30.00 per
month. I wonder if you would consider sending me the full text by Email.
Perhaps you would consider this as an act of friendship between our two
countries.Thank you for your kind consideration of this request
Sincerely: ___________（您的名字）
My Email address is: ____________________ （你的email地址）
最好前面两个，我都要过不错，大家可试试。
这是这一年来的收获，与大家共享，共勉。
－－－－－feat
--
古典狮和时尚兔
※ 来源:·荔园晨风BBS站 bbs.szu.edu.cn·[FROM: 192.168.100.100]
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